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catalytic subunit alpha  (Proteintech)


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    Structured Review

    Proteintech catalytic subunit alpha
    Catalytic Subunit Alpha, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/24503+1+ap/PRKACA+Antibody/pm41698761-72-14-22
    Average 94 stars, based on 32 article reviews
    catalytic subunit alpha - by Bioz Stars, 2026-09
    94/100 stars

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    Western Blot:

    Article Title: ZMYND12 serves as an IDAd subunit that is essential for sperm motility in mice.
    Article Snippet: .. Purchased antibodies included anti-ZMYND12 used for IP, WB and IF (Proteintech, 25587-1-AP), anti-PRKACA used for WB and IF (Proteintech, 24503-1-AP), anti-TTC29 used for WB and IF (Atlas Antibodies, HPA061473), anti-Phosphotyrosine used for WB (Merck Millipore, 05-1050X), anti-AC-TUBULIN used for WB and IF (FineTest, FNab00082), anti-DNAH1 used for IF (Thermo Fisher Scientific, PA5-57826), anti-DNAH2 used for IF (Novus, NBP2-49506), anti-DNAH10 used for IF (Bioss, bs-11022R), anti-DNAH12 used for IF (Thermo Fisher Scientific, PA5-63952), anti-DNALI1 used for IF (Proteintech, 17601-1-AP), Anti-γH2AX used for IF (Abcam, ab81299), Normal Rabbit IgG used for IP (Cell Signaling Technology, 2729 S), Goat-anti-Mouse IgG (H + L)-HRP used for WB (Beyotime, A0216), Goat-anti-Rabbit IgG (H + L)HRP used for WB (Beyotime, A0208), IPKine HRP, Mouse Anti-Rabbit IgG LCS used for WB (Abbkine, A25022), Donkey-anti-Mouse IgG, Alexa Fluor488 used for IF (Thermo Fisher Scientific, A-21,202), Donkey-anti-Rabbit light/dark cycle) with free food and water access. ..

    Article Title: ZMYND12 serves as an IDAd subunit that is essential for sperm motility in mice
    Article Snippet: .. Purchased antibodies included anti-ZMYND12 used for IP, WB and IF (Proteintech, 25587-1-AP), anti-PRKACA used for WB and IF (Proteintech, 24503-1-AP), anti-TTC29 used for WB and IF (Atlas Antibodies, HPA061473), anti-Phosphotyrosine used for WB (Merck Millipore, 05-1050X), anti-AC-TUBULIN used for WB and IF (FineTest, FNab00082), anti-DNAH1 used for IF (Thermo Fisher Scientific, PA5-57826), anti-DNAH2 used for IF (Novus, NBP2-49506), anti-DNAH10 used for IF (Bioss, bs-11022R), anti-DNAH12 used for IF (Thermo Fisher Scientific, PA5-63952), anti-DNALI1 used for IF (Proteintech, 17601-1-AP), Anti-γH 2 AX used for IF (Abcam, ab81299), Normal Rabbit IgG used for IP (Cell Signaling Technology, 2729 S), Goat-anti-Mouse IgG (H + L)-HRP used for WB (Beyotime, A0216), Goat-anti-Rabbit IgG (H + L)-HRP used for WB (Beyotime, A0208), IPKine HRP, Mouse Anti-Rabbit IgG LCS used for WB (Abbkine, A25022), Donkey-anti-Mouse IgG, Alexa Fluor488 used for IF (Thermo Fisher Scientific, A-21,202), Donkey-anti-Rabbit IgG, Alexa Fluor555 used for IF (Thermo Fisher Scientific, A-31,572), Donkey-anti-Rabbit IgG, Alexa Fluor488 used for IF (Thermo Fisher Scientific, A-21,206), and the anti-AKAP3 used for IF was a gift from Qi’s lab [ ]. ..

    Article Title: ZMYND12 serves as an IDAd subunit that is essential for sperm motility in mice
    Article Snippet: .. Antibodies Page 12/29 Purchased antibodies included anti-ZMYND12 used for IP, WB and IF (Proteintech, 25587-1-AP), antiPRKACA used for WB and IF (Proteintech, 24503-1-AP), anti-TTC29 used for WB and IF (Atlas Antibodies, HPA061473), anti-Phosphotyrosine used for WB (Merck Millipore, 05-1050X), anti-AC-TUBULIN used for WB and IF (FineTest, FNab00082), anti-DNAH1 used for IF (Thermo Fisher Scienti c, PA5-57826), antiDNAH2 used for IF (Novus, NBP2-49506), anti-DNAH10 used for IF (Bioss, bs-11022R), anti-DNAH12 used for IF (Thermo Fisher Scienti c, PA5-63952), anti-DNALI1 used for IF (Proteintech, 17601-1-AP), Anti-γH2AX used for IF (Abcam, ab81299), Normal Rabbit IgG used for IP (Cell Signaling Technology, 2729S), Goat-anti-Mouse IgG (H + L)-HRP used for WB (Beyotime, A0216), Goat-anti-Rabbit IgG (H + L)HRP used for WB (Beyotime, A0208), IPKine HRP, Mouse Anti-Rabbit IgG LCS used for WB (Abbkine, A25022), Donkey-anti-Mouse IgG, Alexa Fluor488 used for IF (Thermo Fisher Scienti c, A-21202), Donkey-anti-Rabbit IgG, Alexa Fluor555 used for IF (Thermo Fisher Scienti c, A-31572), Donkey-antiRabbit IgG, Alexa Fluor488 used for IF (Thermo Fisher Scienti c, A-21206), and the anti-AKAP3 used for IF was a gift from Qi’s lab [42]. ..



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    In Vivo Validation via Animal Experiments. A - B The effects of <t>inoculating</t> <t>GJB2-KD/OE</t> A549 ( A ) and NCI-H1975 ( B ) cells on tumor volume and weight were analyzed in a nude mouse subcutaneous tumor model. C - F ELISA was used to detect the relative ratio of cAMP and the levels of IL-10, TGF-β, and IL-4 in the serum of nude mice inoculated with GJB2-KD/OE A549 (C&E) and NCI-H1975 (D&F) cells. G - H Flow cytometry was performed to determine the M1/M2 polarization status of macrophages in tumor tissues of nude mice inoculated with GJB2-KD/OE A549 ( G ) and NCI-H1975 ( H ) cells. I - J WB was used to measure the protein expression levels of <t>PKA,</t> CREB, and p-CREB in tumor tissues of nude mice inoculated with GJB2-KD/OE A549 ( I ) and NCI-H1975 ( J ) cells. K - L IF was employed to observe the expression and nuclear translocation of PKA in tumor tissues of nude mice inoculated with GJB2-KD/OE A549 ( K ) and NCI-H1975 ( L ) cells. All data are presented as means ± standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test and the student’s t test. Statistically significant differences were indicated by * p < 0.05 and ** p < 0.01
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    In Vivo Validation via Animal Experiments. A - B The effects of inoculating GJB2-KD/OE A549 ( A ) and NCI-H1975 ( B ) cells on tumor volume and weight were analyzed in a nude mouse subcutaneous tumor model. C - F ELISA was used to detect the relative ratio of cAMP and the levels of IL-10, TGF-β, and IL-4 in the serum of nude mice inoculated with GJB2-KD/OE A549 (C&E) and NCI-H1975 (D&F) cells. G - H Flow cytometry was performed to determine the M1/M2 polarization status of macrophages in tumor tissues of nude mice inoculated with GJB2-KD/OE A549 ( G ) and NCI-H1975 ( H ) cells. I - J WB was used to measure the protein expression levels of PKA, CREB, and p-CREB in tumor tissues of nude mice inoculated with GJB2-KD/OE A549 ( I ) and NCI-H1975 ( J ) cells. K - L IF was employed to observe the expression and nuclear translocation of PKA in tumor tissues of nude mice inoculated with GJB2-KD/OE A549 ( K ) and NCI-H1975 ( L ) cells. All data are presented as means ± standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test and the student’s t test. Statistically significant differences were indicated by * p < 0.05 and ** p < 0.01

    Journal: Respiratory Research

    Article Title: GJB2 drives LUAD progression in part via cAMP-mediated M2 macrophage polarization through the PKA-CREB pathway

    doi: 10.1186/s12931-026-03537-1

    Figure Lengend Snippet: In Vivo Validation via Animal Experiments. A - B The effects of inoculating GJB2-KD/OE A549 ( A ) and NCI-H1975 ( B ) cells on tumor volume and weight were analyzed in a nude mouse subcutaneous tumor model. C - F ELISA was used to detect the relative ratio of cAMP and the levels of IL-10, TGF-β, and IL-4 in the serum of nude mice inoculated with GJB2-KD/OE A549 (C&E) and NCI-H1975 (D&F) cells. G - H Flow cytometry was performed to determine the M1/M2 polarization status of macrophages in tumor tissues of nude mice inoculated with GJB2-KD/OE A549 ( G ) and NCI-H1975 ( H ) cells. I - J WB was used to measure the protein expression levels of PKA, CREB, and p-CREB in tumor tissues of nude mice inoculated with GJB2-KD/OE A549 ( I ) and NCI-H1975 ( J ) cells. K - L IF was employed to observe the expression and nuclear translocation of PKA in tumor tissues of nude mice inoculated with GJB2-KD/OE A549 ( K ) and NCI-H1975 ( L ) cells. All data are presented as means ± standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test and the student’s t test. Statistically significant differences were indicated by * p < 0.05 and ** p < 0.01

    Article Snippet: Samples were incubated overnight (4°C) with fluorescent primary antibodies: GJB2 (ab303498, Abcam), PKA (27398-1-AP, Proteintech).

    Techniques: In Vivo, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Expressing, Translocation Assay, Standard Deviation